Journal: European Journal of Medical Research

Article Title: The role of CSTF2 in gastric cancer: implications for therapy

doi: 10.1186/s40001-025-03570-8

Figure Lengend Snippet: CSTF2 is abundantly present in gastric cancer. A Pancancer analysis showed that CSTF2 was highly expressed across diverse types of cancers, gastric cancer included. B Immunohistochemistry analysis of CSTF2 in gastric cancerous tissues and regular tissues. C CSTF2 RNA expression degrees in gastric cancer tissues and healthy specimens were compared. D OS/DFS curves analyzed by Kaplan–Meier method; differences assessed by log-rank test

Article Snippet: Antibodies against CSTF2 (1:1000; sc-374373; Santa Cruz Biotechnology, USA) and TGM2 (1:1000; sc-21733; Santa Cruz Biotechnology, USA) were used.

Techniques: Immunohistochemistry, RNA Expression

Journal: European Journal of Medical Research

Article Title: The role of CSTF2 in gastric cancer: implications for therapy

doi: 10.1186/s40001-025-03570-8

Figure Lengend Snippet: CSTF2 drives the expansion of gastric cancer tissues. A WB was used to assess CSTF2 protein degrees in gastric cancer specimens. B Proliferation patterns of BGC-823 and MKN-28 cells. C Assay for colony formation of BGC-823 and MKN-28 cells. D Tumour images and data obtained after stable transfection of gastric cancer specimens with shNC or shCSTF2 and xenografting. All the experiments were replicated at least thrice. A two-tailed independent t test was used. * P < 0.05 and ** P < 0.01. The number of technical replicates for cell experiments is ≥ 3

Article Snippet: Antibodies against CSTF2 (1:1000; sc-374373; Santa Cruz Biotechnology, USA) and TGM2 (1:1000; sc-21733; Santa Cruz Biotechnology, USA) were used.

Techniques: Stable Transfection, Two Tailed Test

Journal: European Journal of Medical Research

Article Title: The role of CSTF2 in gastric cancer: implications for therapy

doi: 10.1186/s40001-025-03570-8

Figure Lengend Snippet: CSTF2 promotes migration and invasion of gastric cancer cells. A Cell migration assay of BGC-823 and MKN-28 cells. B Cell invasion assay of BGC-823 and MKN-28 cells. All experiments were independently repeated at least three times. Statistical significance was determined by a two-tailed independent t test (** P < 0.01)

Article Snippet: Antibodies against CSTF2 (1:1000; sc-374373; Santa Cruz Biotechnology, USA) and TGM2 (1:1000; sc-21733; Santa Cruz Biotechnology, USA) were used.

Techniques: Migration, Cell Migration Assay, Invasion Assay, Two Tailed Test

Journal: European Journal of Medical Research

Article Title: The role of CSTF2 in gastric cancer: implications for therapy

doi: 10.1186/s40001-025-03570-8

Figure Lengend Snippet: CSTF2 regulates the expression of TGM2. A Volcano plot displays gene expression changes following CSTF2 inhibition. B GO analysis of differentially expressed genes after CSTF2 knockdown. C KEGG analysis of genes with changed expression intensity after CSTF2 knockdown

Article Snippet: Antibodies against CSTF2 (1:1000; sc-374373; Santa Cruz Biotechnology, USA) and TGM2 (1:1000; sc-21733; Santa Cruz Biotechnology, USA) were used.

Techniques: Expressing, Gene Expression, Inhibition, Knockdown

Journal: European Journal of Medical Research

Article Title: The role of CSTF2 in gastric cancer: implications for therapy

doi: 10.1186/s40001-025-03570-8

Figure Lengend Snippet: Screening of the downstream gene TGM2. A Venn diagram showing the number of downregulated genes after knockdown of CSTF2 in BGC-823 and MKN-28 cells, with 15 genes overlapping. B mRNA expression levels of CSTF2 and TGM2 after CSTF2 knockdown in different groups, as determined by qRT-PCR. C Spearman correlation analysis of the expression levels between CSTF2 and TGM2. D Protein expression levels of CSTF2 and TGM2 after CSTF2 knockdown, as assessed by western blotting. E BGC-823 and MKN-28 cells were treated with actinomycin D (10 ug/ml) and harvested at 0, 2, 4, 6, and 8 h for RNA extraction. TGM2 mRNA levels were measured by qPCR at the different timepoints, using GAPDH as input control. F Representative immunohistochemistry (IHC) images of TGM2 expression in gastric cancer tissues and adjacent normal tissues (magnification: 200x). All experiments were independently repeated at least three times. Statistical significance was determined by a two-tailed independent t test (* P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Antibodies against CSTF2 (1:1000; sc-374373; Santa Cruz Biotechnology, USA) and TGM2 (1:1000; sc-21733; Santa Cruz Biotechnology, USA) were used.

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, RNA Extraction, Control, Immunohistochemistry, Two Tailed Test

Journal: European Journal of Medical Research

Article Title: The role of CSTF2 in gastric cancer: implications for therapy

doi: 10.1186/s40001-025-03570-8

Figure Lengend Snippet: Rescue experiments. A Protein expression amounts of CSTF2 and TGM2. B Proliferation of BGC-823 and MKN-28. C Colony establishment assay of BGC-823 and MKN-28. Each experiment was conducted a minimum of three times to ensure accuracy. A two-tailed independent t test was used. The number of technical replicates for cell experiments is ≥ 3. ** P < 0.01

Article Snippet: Antibodies against CSTF2 (1:1000; sc-374373; Santa Cruz Biotechnology, USA) and TGM2 (1:1000; sc-21733; Santa Cruz Biotechnology, USA) were used.

Techniques: Expressing, Two Tailed Test